FAA regulated employees, which include, but are not limited to air traffic controllers, aircraft maintenance personnel, aircraft dispatchers, air tour operators, ground security, flight instructors, flight crews, and flight attendants.

For oil and gas companies working on pipeline, which includes operations, maintenance and emergency response, operators and their contractors are required to submit to DOT compliant testing.

Some examples of FMCSA regulated positions: drovers of vehicles required to display a DOT placard when transporting hazardous material, vehicles that carry over 16 passengers including the driver, and CDL (Commercial Driver’s License) holders who operate commercial motor vehicles that weigh a minimum of 26,001 Lbs.

This includes members of employees of railroads including, Hours of Service Act (HSA) personnel, train and engine, and signal service or train dispatchers. Learn more about FRA Regulation 49 CFR Part 219.. Maintenance of Way (MOW) Employees are also required to be in a FRA MOW drug & alcohol testing program 

An agency of the United States Department of Homeland Security follows DOT regulations for drug and alcohol testing. Regulated employees include boat captains and as well as any crewmembers operating commercial vessels.

This DOT agency includes vehicle mechanics, controllers, operators and armed security. Learn more about the FTA, in particular FTA Regulation 49 CFR Part 655.